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      產品詳情
      • 產品名稱:ABI (ID-Plus)AmpFLSTR Identifiler Plus PCR Amplification Kit

      • 產品型號:4427368
      • 產品廠商:ABI
      • 產品文檔:
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      簡單介紹:
      ABI AmpFLSTR Identifiler Plus(ID-Plus) PCR Amplification Kit 貨號:4427368 200reaction
      詳情介紹:

      Optimized for casework, the AmpFLSTR Identifiler Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence. The new kit includes the same primers and allelic ladder as the widely used Identifiler Kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance on mixtures, and the ability to overcome high levels of PCR inhibition.

      Features of the AmpFLSTR Identifiler Plus PCR Amplification Kit include:

      ? Amplifies the same loci as the Identifiler kit with enhanced performance, data quality, and efficiency for casework samples
      ? Helps significantly improve performance on severely inhibited casework samples through the use of a high performance PCR buffer system and optimized thermal cycling parameters
      ? Yields higher peak heights from low-level samples and minor contributors in mixtures to facilitate interpretation
      ? Enables additional analytical flexibility by utilizing fully-validated 28- and 29-cycle protocols
      ? Streamlines workflow by enabling reduced amplification time
      ? Maintains the small amplicon size range of the Identifiler kit (<360 bp) for recovery of information from degraded samples; works in conjunction with the MiniFiler Kit to help maximize recovery from heavily degraded samples
      ? Maintains primer sequences for genotyping concordance with samples typed with the Identifiler, Identifiler Direct, Profiler Plus, COfiler and SGM Plus kits
      ? Utilizes an improved primer manufacturing process to help significantly reduce artifacts

      Other Improvements and Benefits
      The improvements in the Identifiler Plus kit formulation allow for more rapid amplification, eliminating approximately 1 hour from the amplification step compared to the Identifiler kit. In addition, the Identifiler Plus Master Mix includes AmpliTaq Gold DNA Polymerase, which eliminates 2 tubes and an extra box from the kit packaging, and enables streamlined reaction setup.

      None of the formulation enhancements included in the Identifiler Plus kit have required modifications to the Identifiler Kit’s allelic ladder or 5-dye configuration. This configuration allows for optimal locus spacing and amplicon size ranges no greater than 360 bp, enabling better data recovery from degraded samples as compared to other multiplex systems with significantly larger amplicon size ranges. For heavily degraded samples, the MiniFiler kit has been designed to work in conjunction with the Identifiler Plus kit, utilizing miniSTR technology to help maximize recovery of information.

      The Identifiler Plus kit also maintains primer sequences to enable genotyping concordance for samples previously typed using the Identifiler, Identifiler Direct, Profiler Plus, COfiler or SGM Plus kits, and facilitate comparison with existing DNA databases.

      規格

      擴增子大小
      <360 bp
      適用于(設備)
      310 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, GeneAmp 9600, GeneAmp 9700, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer
      標簽或染料
      FAM, LIZ, NED, PET, VIC
      基質和尺寸標準
      GeneScan 500 LIZ Size Standard, Matrix Standard Set DS-33
      產品線
      AmpFLSTR, Identifiler
      產品類型
      PCR Amplification Kit
      樣品體積
      25 μL?reaction
      運輸條件
      Dry Ice
      目標基因位點
      D21S11, CSF1PO, vWA, D8S1179, TH01, D18S51, D5S818, D16S539, D3S1358, D2S1338, TPOX, FGA, D7S820, D13S317, D19S433
      檢測方法
      Fluorescence
      產品規格
      Tube
      GC-Rich PCR Performance
      High
      聚合酶
      AmpliTaq Gold DNA Polymerase
      Reaction Format
      SuperMix or Master Mix
      樣品類型
      DNA (Genomic), Forensic Sample
      技術
      STR (Short Tandem Repeat) Analysis
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